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BS EN ISO 17601:2025 Soil quality. Estimation of abundance of selected microbial gene sequences by quantitative polymerase chain reaction (qPCR) from DNA directly extracted from soil, 2025
- undefined
- 5.1 DNA
- 5.2 Bacteria
- 5.3 Plasmid
- 5.4 Enzymes
- 5.5 Chemicals
- 5.6 Products for bacterial culture medium
- 5.7 Buffers and reagents
- 7.1 qPCR standard preparation and calibration of qPCR assay (task 1) [Go to Page]
- 7.1.1 General
- 7.1.2 Amplicon design (task 1, step 1)
- 7.1.3 qPCR standard preparation (task 1, step 2)
- 7.1.4 Bacterial isolate DNA, environmental DNA, artificial DNA
- 7.1.5 Calibration of the qPCR (task 1, step 3)
- 7.2 Preparation of soil DNA template and inhibition test (task 2) [Go to Page]
- 7.2.1 General
- 7.2.2 Soil DNA preparation (task 2, step 4)
- 7.2.3 Inhibition test (task 2, step 5)
- 7.3 qPCR assay (task 3) [Go to Page]
- 7.3.1 General
- 7.3.2 qPCR (task 3, step 6)
- 7.4 Validation and analysis of qPCR assay (task 4) [Go to Page]
- 7.4.1 General
- 7.4.2 Validation of the qPCR assay (task 4, step 7)
- 7.4.3 Calculation of the copy number of the gene of interest in the soil DNA extract (task 4, step 8) [Go to Page]
