eLibrary >
ISO 20227:2017 Water quality - Determination of the growth inhibition effects of waste waters, natural waters and chemicals on the duckweed Spirodela polyrhiza - Method using a stock culture independent microbiotest >
ISO 20227:2017 Water quality - Determination of the growth inhibition effects of waste waters, natural waters and chemicals on the duckweed Spirodela polyrhiza - Method using a stock culture independent microbiotest, 2017
- Foreword
- Introduction
- 1 Scope
- 2 Normative references
- 3 Terms and definitions
- 4 Principle
- 5 Test organisms
- 6 Growth medium
- 6.1 Preparation of stock solutions
- 6.2 Preparation of the final concentration of modified Steinberg medium
- 7 Apparatus
- 8 Reference chemicals
- 9 Procedure
- 9.1 Germination of the Spirodela polyrhiza turions
- 9.2 Tests on effluents (and waste waters)
- 9.2.1 Addition of concentrated growth medium to the effluent sample
- 9.2.2 Preparation of the effluent dilutions
- 9.2.3 Procedure
- 9.3 Tests on chemical compounds
- 9.3.1 Range finding test
- 9.3.2 Definitive test
- 9.4 Filling of the test plate with the toxicant dilutions
- 9.4.1 General
- 9.4.2 Procedure
- 9.5 Transfer of the germinated turions in the test cups
- 9.6 Photo of the multiwell at the start of the toxicity test
- 9.7 Incubation of the multiwell
- 9.8 Photo of the multiwell at the end of the toxicity test
- 9.9 Measurement of the area of the first fronds
- 10 Data treatment — Calculation of the growth inhibition
- 11 Validity criterion
- 12 Test sensitivity
- 13 Test with reference chemicals
- 14 Test report
- Annex A (informative) Spirodela polyrhiza stock culturing for turion production
- Annex B (informative) Sensitivity of the Spirodela polyrhiza microbiotest
- Annex C (informative) Performance data
- Bibliography [Go to Page]