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ISO/TS 21362:2018 Nanotechnologies - Analysis of nano-objects using asymmetrical-flow and centrifugal field-flow fractionation >
ISO/TS 21362:2018 Nanotechnologies - Analysis of nano-objects using asymmetrical-flow and centrifugal field-flow fractionation, 2018
- Foreword
- Introduction
- 1 Scope
- 2 Normative references
- 3 Terms and definitions
- 4 Symbols and abbreviated terms
- 5 Principles of operation
- 5.1 Field-flow fractionation (General)
- 5.2 Specific applications by applied field
- 5.2.1 Flow field
- 5.2.2 Centrifugal field
- 6 Method development for AF4
- 6.1 General
- 6.2 Sample specifications
- 6.3 Mobile phase specifications
- 6.4 Fractionation
- 6.4.1 Channel and membrane selection
- 6.4.2 Injection and relaxation
- 6.4.3 Optimizing flow conditions
- 6.4.4 Elution programme
- 6.4.5 Using FFF theory to select initial flow settings
- 7 Method development for CF3
- 7.1 General
- 7.2 Choice of mobile phase
- 7.3 Field strength selection
- 7.4 Field decay programme
- 7.5 Channel flow rate selection
- 7.6 Calculation of the relaxation time
- 7.7 ​Calculation of sample injection delay
- 8 Analysis of nano-objects
- 8.1 General
- 8.2 Online size analysis
- 8.3 Online concentration analysis
- 8.3.1 General
- 8.3.2 Mass-based methods
- 8.3.3 Number-based methods
- 8.4 Online material identification or composition
- 8.5 Off-line analysis (Fraction collection)
- 9 Qualification, performance criteria and measurement uncertainty
- 9.1 System qualification and quality control
- 9.1.1 Basic system qualification
- 9.1.2 Focusing performance
- 9.1.3 Flow rate of the carrier liquid
- 9.1.4 Separation field
- 9.2 Method performance criteria
- 9.2.1 Recovery
- 9.2.2 Selectivity
- 9.2.3 Retention
- 9.2.4 Resolution
- 9.3 Method precision and measurement uncertainty
- 10 General procedures for measurement of samples
- 10.1 General
- 10.2 Calibration of retention time for online size analysis
- 10.2.1 Calibration of the AF4 channel
- 10.2.2 Calibration of AF4 retention time for online size measurements
- 10.3 AF4 general measurement procedure
- 10.4 CF3 general measurement procedure
- 11 Test report
- 11.1 General
- 11.2 Apparatus and measurement parameters
- 11.2.1 AF4 recording/reporting specifications
- 11.2.2 CF3 recording/reporting specifications
- 11.3 Reporting test results
- Bibliography [Go to Page]